Biotechnology (Cambridge O Level Biology)

Topic Questions

1
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Which of the following is a reason yeast is used to make bread?

Yeast is used to make bread because...

  • ... it produces ethanol.

  • ... it produces lactic acid.

  • ... it produces carbon dioxide.

  • ... it produces oxygen.

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2
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What is the name of the enzyme used to produce clear apple juice?

  • Lipase

  • Amylase

  • Protease

  • Pectinase

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3
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Why are bacteria used in biotechnology?

  • They can make complex molecules.

  • They are found inside the human body.

  • They reproduce slowly.

  • They can become resistant to antibiotics.

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41 mark

Bacteria are involved in the manufacture of which product? 

  • alcohol

  • bread

  • yoghurt

  • penicillin

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The following statements describe biofuel.

  1. Yeast respires to produce ethanol and carbon dioxide
  2. It is a renewable energy source since it is made from living organisms instead of fossil fuels
  3. Waste parts of crop plants can be used to produce biofuels
  4. In some parts of the world, crops are grown specifically for use as biofuels

Which of these statements could be considered an advantage of biofuel?

  • 1, 2 and 4 

  • 2 and 3 only

  • 2 only

  • 2 and 4 only

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2
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Industrial fermenters are used to produce large quantities of microorganisms which can be used for many biotechnological processes.

Which of the following correctly matches the different conditions in the fermenters and the reason why it is important to control these conditions?

  aseptic conditions optimum pH agitation
A creates an even distribution of substances throughout the fermenter prevents enzymes from denaturing so that enzyme activity is at a maximum ensures that only the desired species of microorganisms grow in the fermenter
B ensures that only the desired species of microorganisms grow in the fermenter creates an even distribution of substances throughout the fermenter prevents enzymes from denaturing so that enzyme activity is at a maximum
C prevents enzymes from denaturing so that enzyme activity is at a maximum ensures that only the desired species of microorganisms grow in the fermenter creates an even distribution of substances throughout the fermenter
D ensures that only the desired species of microorganisms grow in the fermenter prevents enzymes from denaturing so that enzyme activity is at a maximum creates an even distribution of substances throughout the fermenter

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Which of the following combinations of nutrients would be needed in a batch fermenter in the production of penicillin?

  • Amino acids and lipids

  • Lipids and glucose

  • Chlorine and amino acids

  • Glucose and amino acids

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2
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Which products of fermentation (anaerobic respiration in yeast) are important in the production of beer and bread?

  beer bread
A ethanol simple sugars
B lactic acid ethanol
C ethanol carbon dioxide
D simple sugars lactic acid

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3
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Penicillin is produced in a fermenter by growing the fungus Penicillium

The graph below shows how the mass of living Penicillium fungus, and the concentration of penicillin, changed over time.

NYuqN9-n_picture1

Which day would be the best to harvest the penicillin at?

  • 3 days

  • 1.5 days

  • 5 days

  • 3.5 days

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4
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Which food shown in the graph below has the highest percentage stain removal with a biological washing powder?

image-2-1

  • Salad dressing

  • Chocolate pudding

  • Chocolate milkshake

  • Raspberry smoothie

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