Edexcel International A Level Biology

Topic Questions

Gene Technology

1a4 marks

Vitamin A is an essential component of the human diet.

Rice is a staple food in parts of the world where a lot of people have vitamin A deficiency.

Rice contains no β‐carotene or vitamin A.

Cells in the human intestine can convert β‐carotene from the diet to vitamin A.

Some soil bacteria can produce large quantities of β‐carotene.

Describe how genetic engineering could be used to produce rice that contains β‐carotene.

1b4 marks

(b)

(i)
Describe the possible risks of growing genetically modified rice plants.
(3)
(ii)
Suggest how one of these risks could be reduced.
(1)

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2a
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3 marks

The scientific document you have studied is adapted from an article in Science Direct: Assessment of food toxicology by Alexander Gosslau.

Use the information from the scientific article and your own knowledge to answer the following questions.

Explain how toxicants in food could cause Parkinson’s disease (paragraph 3).

2b
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2 marks

Suggest how the genes affected by toxicants can be identified (paragraph 4).

2c
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3 marks

Explain how scientists could determine the high degree of homology of genes between humans and zebrafish (paragraph 5).

2d
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3 marks

Suggest how electrophilic species (ES) of free radicals could be “involved in the modulation of gene expression” (paragraph 6).

2e
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3 marks

Suggest how ES can damage mtDNA (paragraph 7).

2f
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4 marks

In the MTT assay, reduced NAD converts an MTT solution from yellow to purple.

Describe how the MTT assay could be used to show which of two drugs is more toxic to cells (paragraph 10).

2g
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2 marks

Explain the advantages of reprogramming ordinary somatic cells to behave like embryonic stem cells for use in stem cell‐based assays of toxicants (paragraph 11).

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