AQA A Level Chemistry

Revision Notes

7.11.2 Thin Layer Chromatography

Thin Layer Chromatography

  • Thin Layer Chromatography (TLC) is a technique used to analyse small samples via separation
    • For example, we could separate a dye out to determine the mixture of dyes in a forensic sample
  • There are 2 phases involved in TLC – stationary phase and mobile phase
  • Stationary phase
    • This phase is commonly thin metal sheet coated in alumina (Al2O3) or silica (SiO2)
    • The solute molecules adsorb onto the surface
    • Depending on the strength of interactions with the stationary phase, the separated components will travel particular distances through the plate
    • The more they interact with the stationary phase, the more they will ‘stick’ to it
  • Mobile phase
    • Flows over the stationary phase
    • It is a polar or nonpolar liquid (solvent) or gas that carries components of the compound being investigated
    • Polar solvents – water or alcohol
    • Non-polar solvents – alkanes
  • If the sample components are coloured, they are easily identifiable
  • We can examine the plate under UV light using ninhydrin to identify uncoloured components

Conducting a TLC analysis

  • Step 1:
    Prepare a beaker with a small quantity of solvent
  • Step 2:
    On a TLC plate, draw a horizontal line at the bottom edge (in pencil)
    This is called the baseline
  • Step 3:
    Place a spot of pure reference compound on the left of this line, then a spot of the sample to be analysed to the right of the baseline and allow to air dry
    The reference compounds will allow identification of the mixture of compounds in the sample
  • Step 4:
    Place the TLC plate inside the beaker with solvent – making sure that the pencil baseline is lower than the level of the solvent – and place a lid to cover the beaker
    The solvent will begin to travel up the plate, dissolving the compounds as it does
  • Step 5:
    As solvent reaches the top, remove the plate and draw another pencil line where the solvent has reached, indicating the solvent front
    The sample’s components will have separated and travelled up towards this solvent front

Analytical Techniques - TLC Apparatus, downloadable AS & A Level Chemistry revision notes

A dot of the sample is placed on the baseline and allowed to separate as the mobile phase flows through the stationary phase; The reference compound/s will also move with the solvent

Rf values

  • A TLC plate can be used to calculate Rf values for compounds

Thin Layer Chromatography Basics equation

  • These values can be used alongside other analytical data to deduce composition of mixtures

Analytical Techniques - Calculating Rf Values, downloadable AS & A Level Chemistry revision notes

Rf values can be calculated by taking 2 measurements from the TLC plate

Exam Tip

The baseline on a TLC plate must be drawn in pencil. Any other medium would interact with the sample component and solvents used in the analysis process.


Join Save My Exams

Download all our Revision Notes as PDFs

Try a Free Sample of our revision notes as a printable PDF.

Join Now
Already a member?
Go to Top