AQA A Level Biology

Topic Questions

5.4 Nutrient Cycles (A Level only)

1a2 marks

State two roles of nitrogen in living organisms. 

1b3 marks

Figure 1 below shows a representation of the nitrogen cycle.

Figure 1

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Identify the processes or substances occurring at the positions marked A-C in Figure 1.

1c2 marks

Describe the process of ammonification shown in Figure 1.

1d1 mark

The process of denitrification shown in Figure 1 involves anaerobic bacteria. Suggest how flooding might impact the availability of nitrates in the soil.

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2a3 marks

Figure 1 below shows a representation of the phosphorus cycle.

Figure 1

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Identify the processes taking place at the stages marked A-C in Figure 1.

2b2 marks

Describe the role of microorganisms in the phosphorus cycle.

2c2 marks

The waste (faeces and urine) produced by sea birds is also known as guano, and is especially valued as a fertiliser. Use the information in Figure 1 to suggest why this is the case.

2d1 mark

State one role of phosphorus in living organisms.

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3a2 marks

Define the term saprobiont.

3b1 mark

Explain why decomposers are an essential part of any ecosystem.

3c2 marks

Figure 1 below shows a representation of a mycorrhizal relationship between a fungus and a plant root. 

Figure 1

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Explain the importance of this relationship to both the fungus and the plant.

3d1 mark

Some farmers and gardeners are keen to encourage the use of no-dig, or no-till soil management methods. These methods involve disturbing the soil as little as possible, as opposed to more traditional methods that involve ploughing and turning the soil.

Suggest one advantage of no-dig or no-till soil management.

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4a1 mark

A student wanted to investigate the effect of mineral deficiency on the growth of plants. They set up an experiment as shown in Figure 1 below.

Figure 1

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Tube Nutrient solution contents
A Nitrate, phosphate, magnesium, and calcium ions
B Same as A, but no nitrate ions
C Same as A, but no phosphate ions
D Same as A, but no magnesium ions
E Same as A, but no calcium ions

Plants make their own carbohydrates during photosynthesis. Explain why plants also need mineral nutrients such as those mentioned in Figure 1.

4b2 marks

The student set up the experiment shown in Figure 1 so that all test tubes received the same light intensity and were at the same temperature. Explain one other measure shown in Figure 1 that ensures that any results gained are due to mineral deficiency and not other factors.

4c1 mark

The student’s results are shown in Table 1 below.

Table 1

Tube Observations
A Healthy growth
B Stunted growth and yellowing leaves
C Slower growth and patches of dead   tissue on leaves
D Stunted growth and yellowing leaves
E Stunted growth and a weak stem

 

These results are qualitative rather than quantitative. State what is meant by qualitative results.

4d2 marks

Plants need magnesium to make chlorophyll. Use this information and your own knowledge to explain the result for test tube D given in Table 1.

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5a2 marks

Explain why fertilisers are not required in natural ecosystems but are needed in agricultural systems.

5b2 marks

Regular use of fertilisers on farmland can lead to problems such as eutrophication, shown in Figure 1 below.

Figure 1

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Describe the events that have taken place at the positions marked 1 and 4 in Figure 1.

5c1 mark

Name the process carried out by decomposers that leads to stage 5 in Figure 1.

5d2 marks

Fertilisers are essential for most farmers to prevent soils from becoming deficient in minerals. Explain how the problem of eutrophication shown in Figure 1 can be reduced while still maintaining soil mineral availability.

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1a4 marks

A suitable soil for agriculture ensures nutrient cycling for optimum plant growth for agricultural production. A farm’s ability to produce good crop yields is often limited by available soil nutrients, especially nitrogen. Nitrogen is not present at high levels in soil material despite the fact that nitrogen constitutes about 78% of all the atmospheric gases. 

Outline four ways in which nitrogen and its compounds are introduced into soil as a mineral nutrient for plants.

1b2 marks

Nitrifying bacteria in soil convert ammonia into nitrites and nitrates. Researchers examined two soils with different pH for the effect on the communities of nitrifying bacteria found in these soils. They sampled soil from two sites, X and Y.

The pH of the soil samples were:

  • Site X - pH 6.9
  • Site Y - pH 4.3

Ammonia concentrations were measured in the soil samples for the subsequent 20 days. Each sample contained the same concentration of ammonia at the beginning of the experiment and had the same mass. They recorded the ammonia concentrations in:

  • Soil X alone
  • Soil Y alone
  • A mixture of equal masses of soils X and Y with its pH adjusted to 6.9

Their results are shown in Figure 1.

Figure 1

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Suggest why the researchers chose the units of ammonia concentration as shown in Figure 1

1c3 marks

Calculate the difference in the mean rate of breakdown of ammonia per day in the first three days in soil X and soil Y.

Show your working and the units for your answer.

1d3 marks

The researchers concluded that the mixing of soils demonstrated that there were different communities of bacteria in soils X and Y.

Use Figure 1 to provide evidence to support their conclusions. Give reasons for your suggestions.

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2a3 marks

Farmers regularly plough their arable fields. Use your knowledge of the nitrogen cycle to explain the benefits of this practice.

2b3 marks

Describe the nature of mycorrhizae and their role in facilitating the uptake of water and inorganic ions by plants. 

2c3 marks

In the phosphorus cycle, outline three ways in which phosphorus is introduced into soil and into water in oceans, lakes and rivers

2d4 marks

Figure 1 shows the measured phosphate levels in river water upstream and downstream of a large US city during the period 1980 - 1995. The city is surrounded by productive arable land in all directions.  

Figure 1

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In 1990, the city authorities introduced restrictions on the use of phosphorus detergents in the city. 

Explain the effect of these restrictions and suggest an additional measure the authorities can take to further reduce phosphate levels in river water.  

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3a2 marks

The bison is a large, herbivorous mammal that lives on the prairies and plains of North America. Their diet is varied, consisting largely of a mixture of grasses and sedges. Rainfall is high in April to May and also in November. A dry season runs from July to October, during which time rainfall is very low.

Figure 1 shows the pattern of the protein content of all the plants that could form part of the bisons’ diet. Also shown is the protein content of the food the bison actually eat.

Figure 1

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In periods of zero or low rainfall, the plants’ protein content reduces. Suggest one way in
which zero or low rainfall could account for this change.

3b2 marks

At all times of the year, the mean protein content of the food actually eaten by the bison differs from that of the plants which could be eaten. 

Suggest one explanation for this difference.

3c2 marks

Referring to Figure 1, deduce the months in which the difference between protein available and protein eaten is at its minimum and maximum levels. Account for these differences.   

3d2 marks

At times when bison eat food containing less than 5% protein, the protein content of their body tissues has been observed to decrease. 

Suggest and explain how a lack of one named protein makes the bison less able to avoid predation by, for example, wolves.

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4a2 marks

Lentil plants are leguminous and have nodules on their roots which contain bacteria that are able to fix nitrogen. Figure 1 shows some of the processes involved in nitrogen fixation by these bacteria.

Figure 1

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Name substances P and Q.

4b2 marks

The bacteria in the root nodules respire anaerobically. This produces hydrogen and ATP used in nitrogen fixation. The hydrogen comes from reduced NAD. 

Explain how the regeneration of NAD allows ATP production to continue.

4c2 marks

Other than planting leguminous crops and spreading artificial fertilisers, describe andexplain how one farming practice results in the addition of nitrogen-containing compounds to a field.

4d4 marks

Describe and explain how two different farming practices result in the removal of nitrogen-containing compounds from a field.

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5a2 marks

Certain species of nitrogen-fixing bacteria (e.g. Azotobacter and Cyanobacter species) are free-living soil organisms that are not associated with plants mutualistically. As such,they need to find a different source of energy. 

Suggest two means by which these free-living bacteria could generate the energy required for nitrogen fixation.

5b3 marks

One method of sewage treatment is called activated sludge, in which organic matter is fed, as untreated sewage, to bacteria in the treatment tank. These bacteria include decomposers and nitrifying bacteria. A food chain exists within the tank, in which the bacteria are eaten by ciliated protoctists, which are, in turn, eaten by carnivorous protoctists.

Explain the roles of the decomposers and the nitrifying bacteria in converting nitrogen from organic compounds in the sewage into a soluble, inorganic form.

5c3 marks

Describe the importance to ecosystems of the role played by nitrogen-fixing bacteria in the nitrogen cycle.

5d3 marks

Leaching of nitrate in groundwater, often found in run-offs from arable farms, can lead to environmental problems such as eutrophication of ponds, waterways and lakes. Farmers wishing to alleviate this problem have sought to capture their agricultural run-off and treat it as a form of sewage. The treatment employs another group of bacteria to take advantage of a process which occurs naturally in the nitrogen cycle.  Suggest which process this is and provide reasons for your suggestion.

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1a2 marks

The diagram in Figure 1 shows a stream that runs past a farm. A group of students took water samples at various locations along the stream and measured the nitrate concentration in parts per million (ppm) in those water samples. The students have marked these nitrate concentrations on the diagram in Figure 1.

Figure 1

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Explain how the farm may be causing the increase in nitrate concentration in the water of the stream, after it has flowed past the farm.

1b5 marks

The students have been told that nitrate concentration above 200ppm encourage eutrophication in the stream.

Describe the processes that may be occurring in the stream in Figure1 just after it has passed the farm

1c3 marks

Explain why there is increased algal growth in the section of the stream in Figure 1 just after it has flowed past the farm.

1d2 marks

The students investigating the stream in Figure 1 compared the oxygen levels of the water in the stream before it had passed the farm (upstream) and the water of the stream after it had passed the farm (downstream). They found that the water downstream of the farm had a significantly lower oxygen concentration. The students hypothesised that the low oxygen concentration might change the community of organisms in this section of the stream.

What effect(s) might these low oxygen levels have on the community in the water downstream of the farm.

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2a3 marks

Scientists have been monitoring the phosphate concentration in the water of Loch Lomond in Scotland since 1990. The results are shown in the graph in Figure 1.

Figure 1

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Considering Figure 1, give:

i)
One possible source of the phosphate in the Loch.

ii)
The percentage increase in phosphate between 2000 and 2020.
2b3 marks

In 1990 the phosphate levels in the water of Loch Lomond (see Figure 1) were considered optimal for the growth of aquatic plants.

Explain why phosphates are essential for plants to grow.

2c2 marks

Herbivores living in and on Loch Lomond, such as fish and wading birds, also require phosphorus. Explain how herbivores obtain phosphorus from their food.

2d3 marks

Once phosphorus (in the form of phosphates) has been absorbed and used by animals, how does the phosphorus get recycled back into the lake ecosystem of Loch Lomond?

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3a3 marks

The diagram in Figure 1 shows part of the nitrogen cycle occurring on a farmed field where crops are being grown.

Figure 1

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Name the processes labelled X, Y and Z in the diagram in Figure 1.

3b2 marks

Describe how the saprobionts in Figure 1 obtain their nutrients from dead organisms and animal waste products.

3c5 marks

As seen in Figure 1, a key step in the nitrogen cycle that is essential for successful growth of crops is the take up of nitrogen compounds from the soil by the plants. Saprobionts release nitrogen into the soil in the form of ammonia/ammonium ions but another key process must occur before the crops can use this nitrogen.

i)
Name this process.

ii)
Describe this process.
3d2 marks

Adding inorganic fertilisers to a field, as in Figure 1, is a farming practice that can increase the amount of nitrogen-containing compounds in the soil. Describe and explain one other farming practice that can increase the amount of nitrogen-containing compounds in the soil.

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4a2 marks

Two fields, C and D, were used to grow corn. Each field was divided into six plots. Different masses of fertiliser containing ammonium nitrate were applied to each plot. After one month, samples of corn plants were collected from each plot and their mass calculated. The results are shown in the table in Figure 1.

Figure 1

Mass of ammonium nitrate added / kg ha–1

Mass of corn / kg m–2

Field C - used for grazing sheep in previous year

Field D - used for same crop in previous year

0 16.5 5.4
20 17.2 10.3
40 19.1 14.1
60 19.4 16.2
80 19.4 18.5
100 19.4 18.5

Describe the results of the study shown by the table in Figure 1.

4b4 marks

Explain why the mass of corn produced from Field C in Figure 1 increased whenthe mass of fertiliser added was increased from 0 to 40 kg ha–1 but no longerincreased when more than 60 kg ha–1 of fertiliser was added.

4c3 marks

In the year before the investigation in Figure 1 was conducted, Field C had been used for grazing sheep. Field D, however, had been used to grow the same crop as the current year in which this investigation took place. When the mass of fertiliser added was 0 kg ha–1, the mass of corn from Field C was higher than from Field D.

Assuming all other conditions were the same during the investigation, give a possible explanation for this finding.

4d3 marks

Using the results table in Figure 1, determine which field (Field C or Field D) showed the greater percentage increase in mass of corn. State the difference in percentage increase between the two fields?

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5a3 marks

The graph in Figure 1 below shows the effect of adding different amounts of two types of nitrogen-containing fertiliser (fertiliser X and fertiliser Y) on the yield of wheat crops.

Figure 1

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Considering the graph in Figure 1:

i)
The curves for both fertilisers do not start at the origin of the graph. Explain why.

ii)
What mass of nitrogen-only fertiliser (fertiliser Y) would you recommend a farmer to use per square metre for growing wheat? Explain your answer.
5b4 marks

Looking at the curve between 0 and 15 g m-2 for fertiliser Y in Figure 1, it appears that the amount of nitrogen added in the fertiliser is limiting the yield of wheat. Past this point, it appears that something else is limiting the yield.

i)
Using the information in Figure 1, suggest what limits yield after this point.

ii)
The wheat plants receiving fertiliser X can form more ATP and showed a greater yield as a result. Describe the structure of ATP.
5c4 marks

Other than their importance in the production of ATP, state and explain two reasons why phosphate ions are important for the growth of plants.

5d2 marks

Using artificial nitrogen-containing fertilisers, such as in Figure 1, is one way to increase crop yield. There are alternative methods for increasing crop yield, such as including leguminous plants in a crop rotation. Explain how this technique can also improve crop yield.

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