5.2.5 The Krebs Cycle

• The Krebs cycle (sometimes called the citric acid cycle) consists of a series of enzyme-controlled reactions
• 2 carbon (2C) Acetyl CoA enters the circular pathway from the link reaction in glucose metabolism
  • Acetyl CoA formed from fatty acids (after the breakdown of lipids) and amino acids enters directly into the Krebs Cycle from other metabolic pathways

• 4 carbon (4C) oxaloacetate accepts the 2C acetyl fragment from acetyl CoA to form the 6 carbon (6C) citrate
  • Coenzyme A is released in this reaction

• Citrate is then converted back to oxaloacetate through a series of oxidation-reduction (redox) reactions

The Krebs Cycle uses acetyl CoA from the link reaction and the regeneration of oxaloacetate to produce reduced NAD, reduced FAD and ATP

Regeneration of Oxaloacetate

• Oxaloacetate is regenerated in the Krebs cycle through a series of redox reactions
• Decarboxylation of citrate
• • Releasing 2 CO₂ as waste gas

• Oxidation (dehydrogenation) of citrate
  • Releasing H atoms that reduce coenzymes NAD and FAD
  • 3 NAD and 1 FAD → 3NADH + H⁺ and 1 FADH₂

• Substrate-linked phosphorylation
  • A phosphate is transferred from one of the intermediates to ADP, forming 1 ATP